Assessment of microbiological contamination of some household kitchenware

Abstract

This study conducted on  20 samples from four household in Brack region , from five places ( sponges, dishcloths, cutting boards, refrigerator handles and kitchen sink drains), after reaching the samples to the laboratory serial dilutions were made, which cultured on TSA media for total count, After incubation time the growing colonies are counted, morphological studies such as colony morphology, gram stain, shape, size and cell regulation, it conducted for some isolates to evaluate the extent of microbial contamination in some household kitchen tools. The  numbers of microbes on the cutting boards were more than 49x106 cfu/ml, Followed by the handles of refrigerators  estimated at more than 31x106 cfu/ml, , and sponges contained more than 25x106 cfu/ml, and kitchen sink drains containing more than 24x106 cfu/ml, the least in the dish clothes estimated more than 13x106 cfu/ml The total number of isolated strains, The negative is predominant, positive and variable rods respectively, followed by negative, positive and variable cocci, respectively, for the number of isolated strains on the kitchenware, which were more prevalent on dishcloths, sponge, catting boards, refrigerator handles, kitchen sink drains respectively. Micrococcus and Pedicoccus  was found in all samples, Staphylococcus found in the samples of the kitchen sink drains, the dishcloths and the sponges. The bacterium species were identified as the following  Bacillus and curved bacteria (Lactobacillus) found in the sample of the cutting poards and kitchen sink drains, while the constituent of negative bacteria were in the dish clothes samples, cutting poards,handles of refrigerators.