Fungal Biomass Production in Mycological Liquid Medium by Some Ochratoxigenic Fungi Under Static and Shake Culture Conditions
Abstract
In this study, fungal biomass production by some fungi, which were isolated from different cereal samples obtained from the southeast of Libya, was studied by using four mycological medium under shake and static conditions. It has been found that fungal biomass was higher under shake culture than under static conditions. It may be because of the adequate mass and heat transfer. Aspergillus carbonarius isolate produced higher fungal biomass on PDB under shake culture, whereas higher fungal biomass was obtained on MEB under static conditions. By A. ochraceus isolate, it was higher on MEB under shake culture, while it was higher on CPDB under static condition. By A. ochraceus CBS 588.68, it was higher on CPDB under shake culture and on MEB under static conditions. By A. niger 285522, it was higher on MEB under shake culture and on CPDB under static conditions. The results indicate that ochratoxin A production was detected earlier under shake culture than static conditions on the mycological media using the TLC technique.
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References
[1]-AbarcaML,Accensi F, BragulatMR, Cabañes FJ. (2001).Current importance of ochratoxin A-producing Aspergillusspp. J Food Prot;64: 903–6.
[2]-Abarca ML, Accensi F, Cano J, Cabañes FJ. (2004).Taxonomy and significance of black aspergilli.Ant van Leeuwen;86:33–49.
[3]-Abbas, H. K., Microcha, C. J., Meronuk, R. A., Pokorny, J. D., Gould, S. L. and Kommedahl, T. (1988).Mycotoxins and Fusariumspecies associated with infected ears of corn in Minnesota. Applied and Environmental Microbiology,54:1930-1933.
[4]-Battilani P, Pietri A, Bertuzzi AT, Languasco L, Giorni P, Kozakiewicz Z. (2003).Occurrence of ochratoxin A-producing fungi in grapes grown in Italy.J Food Prot;66:633–6.
[5]-Dalcero A, Magnoli C, Hallak C, Chiacchiera SM, Palacio G, DaRocha Rosa CA.(2002). Detection of ochratoxin A in animal feeds and capacity to produce this mycotoxin by Aspergillus section Nigri in Argentina.Food AdditContam;19:1065–72.
[6]-El-Banna, A. A. Pitt, J. I. Leistner, L. (1988).Production of penitrem A by Penicillium crustosumisolated from feedstuffs. International Journal of Food Microbiology, 7:9-17.
[7]-Jeffrey Hurst W, Martin Robert JRA.(1998).High-performance liquid chromatographic determination of ochratoxin A in artificially contaminated cocoa beans using automated sample cleanup. J Chromatogr;810:89–94.
[8]-Krivobok, S., Seigle-Murandi F., Steiman, R. and Marzin, D. (1987).Screening methods to detect toxigenic fungi in liquid medium. Journal of Microbiological Methods,7:29-36.
[9]-Magnoli C, Violante M, Combina M, Palacio G, Dalcero A.(2003).Mycoflora and ochratoxin-producing strains of Aspergillus section Nigri in wine grapes in Argentina. Lett Appl Microbiol;37:179–84.
[10]-Magnoli C, Hallak C, Astoreca A, Ponsone L, Chiacchiera SM, Palacio Gl. (2005).Surveillance of toxigenic fungi and ochratoxin A in feedstuffs from Cordoba province, Argentina. Vet Res Commun;29:431–45.
[11]-Magnoli C, Hallak C, Astoreca A, Ponsone L, Chiacchiera S, Dalcero A.(2006). Occurrence of ochratoxin A-producing fungi in commercial corn kernels in Argentina.Mycopathologia;161:53–8.
[12]-Magnoli C, Astoreca A, Ponsone ML, Fernández-Juri MG, Barberis C, Dalcero A.(2006).Ochratoxin A andAspergillus section Nigri in peanut seeds at different months of storage in Córdoba, Argentina. J Sci Food Agric;86:2369–73.
[13]-Medina A, Jiménez M, Gimeno-Adelantado JV, Valle-Algarra FM, Rufino M.(2005).Determination of ochratoxin A in beer marketed in Spain by liquid chromatography with fluorescence detection using lead hydroxyacetate as a clean-up agent. J. Chromatogra;1083:7-13.
[14]-Pardo E, Marín S, Ramos AJ, Sanchis V. (2004).Occurrence of ochratoxigenic fungi and ochratoxin A in green coffee from different origins. Food Sci Technol Int;10:45–9.
[15]-Rosa CAR, Ribeiro JMM, Fraga MJ, Gatti M, Cavaglieri LR, Magnoli CE.(2006).Mycoflora of poultry feeds and ochratoxin-producing ability of isolated Aspergillusand Penicilliumspecies. Vet Microbiol;113:89–96.
[16]-Samson, R. A., Hokstra, E. S., Frisvad, J. C. and Filteenborg, O. (2002). Methods for detection and isolation in food-born fungi. Introduction to food fungi 6th Ed. Centraalbureau voor Schimmelcultures, Baarn pp 76-77.
[17]-Samson RA, Houbraken JAMP, Kuijpers AFA, Frank JM, Frisvad JC.(2004).New ochratoxin A or sclerotium producing species in Aspergillussection Nigri.Stud Mycol;50:45–61.
[18]-Scott, R. E., Jones, A., Lam, K. S., Gaucher, G. M. (1986). Manganese and antibiotic biosynthesis: I. A specific manganese requirement for patulin production in Penicillium urtcae. Canadian Journal of Microbiology, 32(3):259-267.
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